Reduction in nonfluorescence state of quantum dots on an immunofluorescence staining

Songhua Li-Shishido, Tomonobu M. Watanabe, Hiroshi Tada, Hideo Higuchi, Noriaki Ohuchi

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Fluorescence quantum dots are widely used in immunofluorescence staining because of their intense and stable fluorescence. However, the nonfluorescence state of the quantum dots is their disadvantage. Here, the nonfluorescence state of the dots labeled to cells and tissues was suppressed. Cells and tissues where the receptor HER2 had been overexpressed were fixed and then labeled with anti-HER2 crosslinked with the dots. The intensity of the dots increased with the illumination time. The majority of the single dots were in the nonfluorescence state at beginning of the illumination period and the number of fluorescence dots observed increased with the illumination time. Living cells were also labeled with the anti-HER2-Qdots. Blinking and bleaching of the Qdots was effectively suppressed by adding β-mercaptoethanol and glutathione. Therefore, the movement of the Qdots bound to cell membrane could be observed for long periods of time.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalBiochemical and biophysical research communications
Volume351
Issue number1
DOIs
Publication statusPublished - 2006 Dec 8

Keywords

  • Fixed cell
  • Fixed tissue
  • Imaging
  • Immunofluorescence
  • Live cell
  • Nanotechnology
  • Quantum dots
  • Single molecules
  • Single particles

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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