Reduction by sevoflurane of adenosine 5′-triphosphate-activated inward current of locus coeruleus neurons in pontine slices of rats

Eiji Masaki, Masahito Kawamura, Fusao Kato

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Increasing evidence indicates that volatile general anesthetics exert their effects by affecting various types of membrane conductance expressed in the central nervous system (CNS), such as ligand-gated receptor-channels. The most recently identified family of the receptor-channels in the CNS are the extracellular ATP-gated channels (P2X purinoceptors). In the present study, we tested whether volatile anesthetics can affect P2X receptor function in the CNS network. We recorded whole-cell currents of locus coeruleus (LC) neurons in pontine slices from young rats. Adenosine 5′-triphosphate (ATP) sodium (0.03-3 mM) evoked a rapidly rising and moderately desensitizing inward current (50-200 pA) in a dose-dependent manner in LC neurons at a holding potential of -80 mV. Perfusion with clinically relevant concentration of sevoflurane (0.1-0.5 mM) reduced the ATP-induced inward current in a dose-dependent manner (to 56.8±5.9% of control with 0.5 mM sevoflurane; mean±S.E.M., n=13). Estimated IC50 of sevoflurane was 0.59 mM. We conclude that the attenuation of extracellular ATP-mediated signaling in the central nervous system might be one of the multiple actions of volatile anesthetics.

Original languageEnglish
Pages (from-to)226-232
Number of pages7
JournalBrain research
Volume921
Issue number1-2
DOIs
Publication statusPublished - 2001 Dec 7

Keywords

  • Adenosine 5′-triphosphate
  • Lucos coeruleus
  • P2X receptor
  • Patch-clamp
  • Pontine slice
  • Sevoflurane

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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