Reduced susceptibility to a tobacco bushy top virus malawi isolate by loss of function in host eif(Iso)4e genes

Hisashi Udagawa, Kazuharu Koga, Akira Shinjo, Hiroyasu Kitashiba, Yoshimitsu Takakura

Research output: Contribution to journalArticlepeer-review

Abstract

Tobacco bushy top disease (TBTD) is a viral disease of tobacco (Nicotiana tabacum L.) caused by mixed infection of Tobacco bushy top virus or Ethiopian tobacco bushy top virus and a helper virus. Despite its damage to tobacco, practical genetic resources for disease resistance have not been found. Here, we report that a mutation of tobacco eIF(iso)4E genes (eIF(iso)4E-S and eIF(iso)4E-T), which encode eukaryotic translation initiation factors, confers resistance (reduced susceptibility) to TBTD caused by a virus from Malawi (designated as tobacco bushy top virus Malawi isolate, TBTV-MW). RNAi lines in which eIF(iso)4E genes were silenced showed reduced susceptibility to TBTV-MW. We also tested chemically-induced single (eIF(iso)4E-S or eIF(iso)4E-T) and double (eIF(iso)4E-S and eIF(iso)4E-T) nonsense mutants for resistance to TBTV-MW. Suppression of eIF(iso)4E-S showed reduced susceptibility, and the resistance of the double mutant tended to be even stronger. eIF(iso)4E mutants also showed reduced susceptibility to TBTV-MW transmitted by aphids. To the best of our knowledge, the eIF(iso)4E-S mutant is the first genetic resource for TBTD resistance breeding in tobacco.

Original languageEnglish
Pages (from-to)313-320
Number of pages8
JournalBreeding Science
Volume70
Issue number3
DOIs
Publication statusPublished - 2020

Keywords

  • EIF(iso)4E
  • Nicotiana tabacum
  • Tobacco bushy top virus
  • Translation initiation factor

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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