Reduced sialidase expression in highly metastatic variants of mouse colon adenocarcinoma 26 and retardation of their metastatic ability by sialidase overexpression

Masashi Sawada, Setsuko Moriya, Seiichi Saito, Ryuzaburo Shineha, Susumu Satomi, Takao Yamori, Takashi Tsuruo, Reiji Kannagi, Taeko Miyagi

Research output: Contribution to journalArticlepeer-review

73 Citations (Scopus)

Abstract

Sialidase expression levels are inversely correlated with the metastatic potential of mouse colon adenocarcinoma 26 sublines, as assessed by activity assays and RT-PCR, irrespective of total and cell surface sialic acid contents. Compared with low metastatic NL4 and NL44 cell lines, the highly metastatic NL17 and NL22 cells exhibit low expression of sialidases, accompanied with higher levels of sialylLex and GM3. To investigate whether these properties of NL17 cells can be altered by sialidase overexpression, we transfected a cytosolic sialidase gene into NL17 cells. The result was markedly inhibited lung metastasis, invasion and cell motility with a concomitant decrease in sialylLex and GM3 levels, in line with the case of spontaneously low metastatic sublines having relatively high endogenous sialidase levels, implying that sialidase level is a determining factor affecting metastatic ability. Treatment of the cells with antibodies against sialylLex and GM3 affected cell adhesion and/or cell motility, providing evidence that desialylation of these molecules, as targets of sialidase, is involved in the suppression of metastasis.

Original languageEnglish
Pages (from-to)180-185
Number of pages6
JournalInternational Journal of Cancer
Volume97
Issue number2
DOIs
Publication statusPublished - 2002 Jan 10

Keywords

  • Colon adenocarcinoma
  • Metastasis
  • Sialic acids
  • Sialidase

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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