Reduced HIC-1 gene expression in non-small cell lung cancer and its clinical significance

M. Hayashi, Y. Tokuchi, T. Hashimoto, S. I. Hayashi, K. Nishida, Y. Ishikawa, K. Nakagawa, S. Tsuchiya, S. Okumura, E. Tsuchiya

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35 Citations (Scopus)

Abstract

Background: HIC-1 (hypermethylated in cancer-1) is a candidate tumor suppressor gene, identified in a region of frequent loss of heterozygosity on chromosome 17p13.3, which is telomeric from TP53 and often deleted in surgically resected lung cancers. To determine the significance of HIC-1 in lung cancer, we assessed its expression status and prognostic association in 47 adenocarcinomas and squamous cell carcinomas. Materials and Methods: RNA was extracted from tumors and corresponding normal tissues of surgically resected lungs, and the amount of HIC-1 mRNA was determined by means of semi-quantitative reverse transcriptase-polymerase chain reaction. Results: HIC-1 expression in tumors was less than that in normal lung tissues in 40 of 47 patients (85%), indicating frequent partial silencing. Median tumor/normal lung tissue (T/L) ratios for HIC-1 expression were 0.51 and 0.75 for adenocarcinomas and squamous cell carcinomas, respectively. No significant difference of median T/L ratio was observed between the two histological types, or among clinical stages of the patients. However, the reduced expression of HIC-1 gene in the tumor had a direct link with the clinical outcome: lower T/L ratios (<0.5) were significantly associated with short survival (P=0.034), an association also observed in cases restricted to stage I (P=0.047). Conclusions: The results suggest that low HIC-1 expression is involved in malignant progression of non-small cell lung cancer.

Original languageEnglish
Pages (from-to)535-540
Number of pages6
JournalAnticancer research
Volume21
Issue number1 B
Publication statusPublished - 2001 Apr 17
Externally publishedYes

Keywords

  • Chromosome 17p13.3
  • HIC-1 expression
  • Lung cancer
  • Prognosis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Hayashi, M., Tokuchi, Y., Hashimoto, T., Hayashi, S. I., Nishida, K., Ishikawa, Y., Nakagawa, K., Tsuchiya, S., Okumura, S., & Tsuchiya, E. (2001). Reduced HIC-1 gene expression in non-small cell lung cancer and its clinical significance. Anticancer research, 21(1 B), 535-540.