Redox sensor proteins for highly sensitive direct imaging of intracellular redox state

Kazunori Sugiura, Takeharu Nagai, Masahiro Nakano, Hiroshi Ichinose, Takakazu Nakabayashi, Nobuhiro Ohta, Toru Hisabori

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Intracellular redox state is a critical factor for fundamental cellular functions, including regulation of the activities of various metabolic enzymes as well as ROS production and elimination. Genetically-encoded fluorescent redox sensors, such as roGFP (Hanson, G. T., et al. (2004)) and Redoxfluor (Yano, T., et al. (2010)), have been developed to investigate the redox state of living cells. However, these sensors are not useful in cells that contain, for example, other colored pigments. We therefore intended to obtain simpler redox sensor proteins, and have developed oxidation-sensitive fluorescent proteins called Oba-Q (oxidation balance sensed quenching) proteins. Our sensor proteins derived from CFP and Sirius can be used to monitor the intracellular redox state as their fluorescence is drastically quenched upon oxidation. These blue-shifted spectra of the Oba-Q proteins enable us to monitor various redox states in conjunction with other sensor proteins.

Original languageEnglish
Pages (from-to)242-248
Number of pages7
JournalBiochemical and biophysical research communications
Volume457
Issue number3
DOIs
Publication statusPublished - 2015 Feb 13

Keywords

  • Biosensor
  • Disulfide
  • Fluorescence
  • Oxidation-reduction
  • Redox regulation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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