Redox cycling-based electrochemical reporter gene assay for single cells using a scanning electrochemical microscope-microwell system

Hiroki Ida, Kosuke Ino, Junya Suzuki, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Single-cell analyses are important for providing new insights into cellular biology. Here we report an electrochemical reporter gene assay for single cells using a scanning electrochemical microscope (SECM)-microwell system. Each microwell trapped a single cell that synthesized a reporter protein, secreted alkaline phosphatase (SEAP). The SEAP catalyzed the hydrolysis of p-aminophenyl phosphate to p-aminophenol (PAP). A disk electrode in the SECM was positioned above the microwell and monitored the oxidation currents of PAP derived from SEAP. In addition, ring electrodes were prepared on the microwell device to induce redox cycling between the ring and disk electrodes, thus amplifying the electrochemical signals from the reporter protein. The redox cycling-based electrochemical reporter gene assay is useful for single-cell analyses.

Original languageEnglish
Pages (from-to)308-311
Number of pages4
JournalElectrochemistry
Volume84
Issue number5
DOIs
Publication statusPublished - 2016 May

Keywords

  • Redox cycling
  • Reporter gene assay
  • SECM
  • Single-cell analysis

ASJC Scopus subject areas

  • Electrochemistry

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