Rediscovery of the Yesso scallop pathogen Perkinsus qugwadi in Canada, and development of PCR tests

Naoki Itoh, Gary R. Meyer, Amy Tabata, Geoff Lowe, Cathryn L. Abbott, Stewart C. Johnson

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Perkinsus qugwadi, a pathogenic protozoan parasite of Yesso scallops Patinopecten yessoensis, is found only in cultured populations in British Columbia, Canada. This pathogen was first identified in 1988 and caused significant mortalities at some locations during the early 1990s. Prevalence of infection decreased dramatically following 1995, and the disease was last reported in 1997, leading to speculation that the Yesso scallop stocks in Canada had developed resistance to the disease, or that P. qugwadi had disappeared. However, the present study revealed that infection with P. qugwadi and associated mortality is still occurring in scallops from at least one location in British Columbia. One of the PCR tests developed for P. qugwadi detected the parasite in a 105-fold dilution of DNA extracted from a heavily infected sample and detected 52% more positive scallops than histology; however, the assay also cross-reacted with P. honshuensis and P. olseni. The other PCR test was less sensitive and detected 34% more positives, but did not react to any of the other Perkinsus species tested, suggesting that these PCR tests are powerful tools for screening for the presence of P. qugwadi. Phylogenetic analysis of 1796 bp of SSU rRNA gene sequence clearly indicated that P. qugwadi is positioned basally to other Perkinsus species.

Original languageEnglish
Pages (from-to)83-91
Number of pages9
JournalDiseases of Aquatic Organisms
Volume104
Issue number1
DOIs
Publication statusPublished - 2013 Apr 29

Keywords

  • Bivalves
  • Diagnosis
  • Molecular detection
  • Parasite disease
  • Perkinsus
  • Phylogeny

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Fingerprint Dive into the research topics of 'Rediscovery of the Yesso scallop pathogen Perkinsus qugwadi in Canada, and development of PCR tests'. Together they form a unique fingerprint.

  • Cite this