Rectified photocurrent in a protein based molecular photo-diode consisting of a cytochrome b562-green fluorescent protein chimera self-assembled monolayer

Bumhwan Lee, Shuji Takeda, Ken Nakajima, Jaegeun Noh, Jeongwoo Choi, Masahiko Hara, Teruyuki Nagamune

    Research output: Contribution to journalArticlepeer-review

    28 Citations (Scopus)

    Abstract

    We fabricated a self-assembled monolayer (SAM) of a chimeric protein created as a novel model protein for an artificial light-harvesting complex (LHC) composed of two proteins, cytochrome b562 (cytb562) mutated for SAM fabrication (cytb562, N22C, G82C) and enhanced green fluorescent protein (EGFP). The SAM formation of chimeric protein on a single-crystalline Au(111) substrate was confirmed by atomic force microscopy (AFM) measurement. The rectified photocurrent of the chimeric protein SAM on a gold substrate was detected by light-illumination scanning tunneling microscopy (LI-STM) co-operated with a lock-in technique. The photocurrent generation of the chimeric protein SAM was wavelength-specific to the light-illumination (488nm), which indicated that the EGFP part of the chimera plays a role as a sensitizer in the photo-induced electron transfer process.

    Original languageEnglish
    Pages (from-to)1169-1174
    Number of pages6
    JournalBiosensors and Bioelectronics
    Volume19
    Issue number10
    DOIs
    Publication statusPublished - 2004 May 15

    Keywords

    • Cytochrome b
    • Enhanced green fluorescent protein
    • Light-harvesting complex
    • Light-illumination scanning tunneling microscopy
    • Photocurrent generation
    • Self-assembled monolayer

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Electrochemistry

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