Reconstitution of functional interleukin 2 receptor complexes on fibroblastoid cells: Involvement of the cytoplasmic domain of the γ chain in two distinct signaling pathways

Hironobu Asao, Toshikazu Takeshita, Naoto Ishii, Satoru Kumaki, Masataka Nakamura, Kazuo Sugamura

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

We have previously shown that the interleukin 2 (IL-2) receptor γ chain is a member of the cytokine receptor superfamily and is indispensable for the formation of receptor complexes with high and intermediate affinities for IL-2. The present study demonstrates that the αβγ heterotrimer and βγ heterodimer complexes of IL-2 receptor reconstituted on murine fibroblast L929 cells can transduce IL-2-mediated signals for activation of tyrosine kinase and for induction of c-myc, c-fos, and c-jun expression. A mutant of the γ chain lacking the C-terminal 68 amino acids in its cytoplasmic region showed a loss of such signal-transducing ability when incorporated into the IL-2 receptor complexes but brought no effect on IL-2 binding and IL-2 internalization. Another mutant, with a C-terminal deletion of 30 amino acids, retained the ability to activate a tyrosine kinase and to induce c-myc expression but lost the ability to induce c-fos and c-jun expression. These results suggest that at least two distinct signals, one for c-myc induction, which parallels tyrosine kinase activation, and the other for c-fos and c-jun induction, can be transduced from the IL-2 receptor complexes reconstituted on fibroblastoid cells.

Original languageEnglish
Pages (from-to)4127-4131
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number9
DOIs
Publication statusPublished - 1993 May 1

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Reconstitution of functional interleukin 2 receptor complexes on fibroblastoid cells: Involvement of the cytoplasmic domain of the γ chain in two distinct signaling pathways'. Together they form a unique fingerprint.

  • Cite this