Reconstitution of bacterial photosynthetic unit in a lipid bilayer studied by single-molecule spectroscopy at 5 K

Daisuke Uchiyama, Hiroyuki Oikawa, Kohei Otomo, Mamoru Nango, Takehisa Dewa, Satoru Fujiyoshia, Michio Matsushita

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10 Citations (Scopus)

Abstract

As a model of photosynthetic unit (PSU), self-assembled aggregates of pigmentprotein complexes from photosynthetic bacteria were prepared in a lipid bilayer by reconstitution of the light-harvesting 2 (LH2) complex and light-harvesting 1reaction center (LH1RC) complex through detergent removal of their micelles in the presence of lipids. By performing polarization-controlled fluorescence and fluorescence-excitation spectroscopy on single aggregates at a temperature of 5 K, the composition of individual aggregates was determined and excitation energy transfer (EET) between constituent complexes was observed. LH2 and LH1RC from a bacterium, Rhodobacter (Rb.) sphaeroides, were found to form a trimeric aggregate in which EET takes place from one LH2 to two LH1RCs. In contrast, a heterodimer of LH2 and LH1RC in which EET works was found to assemble from a combination of complexes of different bacterial species, that is, LH2 from Rb. sphaeroides and LH1RC from Rhodopseudomonas (Rps.) palustris.

Original languageEnglish
Pages (from-to)11615-11619
Number of pages5
JournalPhysical Chemistry Chemical Physics
Volume13
Issue number24
DOIs
Publication statusPublished - 2011 Jun 28

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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