Recognition of N-glycosidic carbohydrates on esophageal carcinoma cells by macrophage cell line THP-1

R. Takano, M. Nose, H. Kanno, T. Nishihira, S. Hiraizumi, A. Kobata, M. Kyogoku

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13 Citations (Scopus)

Abstract

Cell-to-cell contact between macrophages and tumor cells is an important initial reaction in a host defense mechanism against tumor cells. The authors have studied cell surface components of human esophageal carcinoma cells recognized by macrophages. Superoxide release from THP-1 cells, a human macrophage cell line, was analyzed in their interaction with a battery of human squamous cell carcinoma cell lines (TE) originated from esophageal cancer patients. The macrophage-triggering ability of TE 1 cell line, a high stimulant, was reduced after treatment with trypsin or tunicamycin, an inhibitor of N-glycosidic glycosylation. Addition of monosaccharides was efficient in competitive inhibition of these cellular interaction. Moreover, con-A-resistant mutation of TE 1 cells was found to reduce their macrophage-triggering ability, associated with increase of L-PHA-binding capacity, suggesting substitution to the GlcNAc β(1 → 6)-linked lactosamine antenna in N-glycosidic carbohydrates. These findings suggest that terminal residues of N-glycosidic carbohydrates on some esophageal carcinoma cells may contribute to the recognition sites of macrophages.

Original languageEnglish
Pages (from-to)393-401
Number of pages9
JournalAmerican Journal of Pathology
Volume137
Issue number2
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Takano, R., Nose, M., Kanno, H., Nishihira, T., Hiraizumi, S., Kobata, A., & Kyogoku, M. (1990). Recognition of N-glycosidic carbohydrates on esophageal carcinoma cells by macrophage cell line THP-1. American Journal of Pathology, 137(2), 393-401.