TY - JOUR
T1 - Reciprocal T cell responses in the liver and thymus of mice injected with syngeneic tumor cells
AU - Seki, Shuhji
AU - Abo, Toru
AU - Sugiura, Keitaro
AU - Ohteki, Toshiaki
AU - Kobata, Tetsuji
AU - Yagita, Hideo
AU - Okumura, Ko
AU - Rikiishi, Hidemi
AU - Masuda, Takayuki
AU - Kumagai, Katsuo
N1 - Funding Information:
’ This work was supported in part by a Grant-in-Aid for Scientific Research and Cancer Research from the Ministry of Education, Science,a nd Culture, and a Grant-in-Aid from the Public Health Bureau, Ministry of Health and Welfare, Japan. 2 To whom correspondence should be addresseda t Toru Abo, Department of Immunology, Niigata University School of Medicine, Niigata 95 1, Japan.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1991/10/1
Y1 - 1991/10/1
N2 - We investigated the T cell responses in various tissues, especially in the liver and thymus, of mice injected with syngeneic tumors. This study was undertaken since recent evidence indicated that the liver is one of the important immune organs for T cell proliferation. When C3H/He mice were intraperitoneally injected with mitomycin-treated syngeneic MH134 tumors (1 × 107/mouse), a transient increase of liver mononuclear cells (MNC) was induced, showing a peak at Day 4 after injection. Histological study of such liver showed a sinusoidal dilatation and an accumulation of MNC in the sinusoids. The most predominant MNC induced were double negative (CD4P-8-) αβ T cells and γδ T cells. These γδ T cells varied, showing unique time-kinetics. Despite a continuous increase of whole liver MNC and αβ T cells, the proportion of γδ T cells in the liver decreased beginning 4 days after injection. In contrast with the response in the liver, a striking decrease in the cell number of thymocytes was induced after tumor injection, showing a basal level at Day 6. This hypocellularity in the thymus appears to be an inverted response of the lymphocytosis in the liver. At this time, a corresponding decrease in the proportion of double positive (CD4+8+) T cells was always seen in the thymus. Analysis of cell proliferative response showed that the increase of liver MNC after tumor injection was accompanied by augmented proliferation, whereas the decrease of thymocytes was accompanied by depressed proliferation. The present results indicate that there exists a unique, reciprocal response of T lymphocytes between the liver and thymus, and that the presence of tumor appears to stimulate T cell response in the liver but alternatively inactivates such response in the thymus.
AB - We investigated the T cell responses in various tissues, especially in the liver and thymus, of mice injected with syngeneic tumors. This study was undertaken since recent evidence indicated that the liver is one of the important immune organs for T cell proliferation. When C3H/He mice were intraperitoneally injected with mitomycin-treated syngeneic MH134 tumors (1 × 107/mouse), a transient increase of liver mononuclear cells (MNC) was induced, showing a peak at Day 4 after injection. Histological study of such liver showed a sinusoidal dilatation and an accumulation of MNC in the sinusoids. The most predominant MNC induced were double negative (CD4P-8-) αβ T cells and γδ T cells. These γδ T cells varied, showing unique time-kinetics. Despite a continuous increase of whole liver MNC and αβ T cells, the proportion of γδ T cells in the liver decreased beginning 4 days after injection. In contrast with the response in the liver, a striking decrease in the cell number of thymocytes was induced after tumor injection, showing a basal level at Day 6. This hypocellularity in the thymus appears to be an inverted response of the lymphocytosis in the liver. At this time, a corresponding decrease in the proportion of double positive (CD4+8+) T cells was always seen in the thymus. Analysis of cell proliferative response showed that the increase of liver MNC after tumor injection was accompanied by augmented proliferation, whereas the decrease of thymocytes was accompanied by depressed proliferation. The present results indicate that there exists a unique, reciprocal response of T lymphocytes between the liver and thymus, and that the presence of tumor appears to stimulate T cell response in the liver but alternatively inactivates such response in the thymus.
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U2 - 10.1016/0008-8749(91)90055-G
DO - 10.1016/0008-8749(91)90055-G
M3 - Article
C2 - 1653116
AN - SCOPUS:0026042431
VL - 137
SP - 46
EP - 60
JO - Cellular Immunology
JF - Cellular Immunology
SN - 0008-8749
IS - 1
ER -