Receptor for activated C kinase 1 stimulates nascent polypeptide-dependent translation arrest

Kazushige Kuroha, Mayuko Akamatsu, Lyudmila Dimitrova, Takehiko Ito, Yuki Kato, Katsuhiko Shirahige, Toshifumi Inada

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Nascent peptide-dependent translation arrest is crucial for the quality control of eukaryotic gene expression. Here we show that the receptor for activated C kinase 1 (RACK1) participates in nascent peptide-dependent translation arrest, and that its binding to the 40S subunit is crucial for this. Translation arrest by a nascent peptide results in Dom34/Hbs1-independent endonucleolytic cleavage of mRNA, and this is stimulated by RACK1. We propose that RACK1 stimulates the translation arrest that is induced by basic amino-acid sequences that leads to endonucleolytic cleavage of the mRNA, as well as to co-translational protein degradation.

Original languageEnglish
Pages (from-to)956-961
Number of pages6
JournalEMBO Reports
Volume11
Issue number12
DOIs
Publication statusPublished - 2010 Dec 1

Keywords

  • RACK1
  • endonucleolytic cleavage
  • mRNA quality control
  • nascent peptide
  • translation arrest

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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