Receptor autoradiography with 11c and [3h]-labelled ligands visualized by imaging plates

Kazuhiko Yanai, Jong Hoon Ryu, Takehiko Watanabe, Ren Iwata, Tatsuo Ido

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

The distribution of histamine H„ H3, dopamine D, and D2 receptors in the brain was studied by receptor autoradiography using a high-sensitivity and high-resolution imaging plate system. [3H]Pyrilamine, [3H](i?)a-methyl-histamine, ["C]SCH23390, and ["QiV-methylspiperone (or ["C]YM-09151-2) were used as ligands to identify H„ H3, D, and D2 receptors, respectively. Two different receptors (dopamine D2 and histamine H3) could be also labelled simultaneously in a single cryostat-sliced section using [nC]iV-methylspiperone and [3H](i?)a-methylhistamine, respectively. The imaging plate system is useful for receptor autoradiography of positron emitter-labelled and tritium-labelled receptor-ligands because of its high sensitivity.

Original languageEnglish
Pages (from-to)961-964
Number of pages4
JournalNeuroReport
Volume3
Issue number11
DOIs
Publication statusPublished - 1992 Nov

Keywords

  • Brain
  • Dopamine D
  • Dopamine D2 receptor
  • Double nuclide autoradiography
  • Histamine H
  • Histamine H3 receptor
  • Imaging plates
  • Positron emission tomography
  • Receptor
  • Receptor

ASJC Scopus subject areas

  • Neuroscience(all)

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