Recent progress in abasic site-binding small molecules for detecting single-base mutations in DNA

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

This review addresses our recent efforts to design AP site-binding small ligands for SNP (single-nucleotide polymorphisms) typing. First, we present a surface plasmon resonance (SPR) biosensor carrying a derivative of 3,5-diaminopyrazines. Comparison with a bulk assay based on 3,5-diaminopyrazines-DNA binding shows that the immobilization of 3,5-diaminopyrazines on the SPR sensor allows a more sensitive detection of the target DNA, and binding selectivity can be tuned by controlling salt concentrations of the sample solutions. We also present a ratiometric fluorescent probe, in which an environmentally sensitive fluorescent dye, a benzofurazan derivative, is linked through an alkyl spacer to a 2-amino-1,8-naphthyridine derivative. The binding and sensing properties of these systems are discussed as a basis for the advanced design of DNA-binding small molecules for gene detection.

Original languageEnglish
Pages (from-to)137-142
Number of pages6
Journalanalytical sciences
Volume30
Issue number1
DOIs
Publication statusPublished - 2014 Jan

Keywords

  • Biosensors
  • DNA recognition
  • Fluorescent probes
  • Ligand design
  • Noncovalent interactions
  • Nucleobases
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Analytical Chemistry

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