TY - JOUR
T1 - Recent advances in renal regeneration [version 1; referees
T2 - 2 approved]
AU - Hasegawa, Sho
AU - Tanaka, Tetsuhiro
AU - Nangaku, Masaomi
N1 - Funding Information:
Grant information: This work was supported by Grant-in-Aid for Scientific Research (B) (JSPS KAKENHI grant 18H02824 to MN), Grant-in-Aid for Scientific Research (C) (JSPS KAKENHI grant 17K09688 to TT), and Grants-in-Aid for Scientific Research on Innovative Areas (KAKENHI grants 26111003 to MN).
Publisher Copyright:
© 2019 Hasegawa S et al.
PY - 2019
Y1 - 2019
N2 - Regeneration of a functional kidney from pluripotent stem cells (PSCs) is challenging because of its complex structure. Kidneys are derived from embryonic metanephros, which are composed of three progenitor cells: nephron progenitors, ureteric bud, and stromal progenitors. Nephron progenitors and ureteric bud have been induced successfully from PSCs as a result of the understanding of their detailed developmental process through cell-lineage tracing analysis. Moreover, these induced progenitors can be used to reconstruct the three-dimensional (3D) structure of kidneys in vitro, including glomeruli with podocytes, renal tubules, and the branching ureters. Induction of the remaining renal progenitors (that is, stromal progenitors from PSCs and the further maturation of reconstructed kidneys) needs to be studied extensively to regenerate functional and sophisticated kidneys from PSCs. In addition to the proper induction of renal progenitors, new bioengineering methods such as decellularization and 3D bioprinting and the recent advancements in the regeneration of kidneys in other species are promising leads for regenerating the complex spatial arrangement of kidneys, including the vascular network and urinary excretion pathway in humans.
AB - Regeneration of a functional kidney from pluripotent stem cells (PSCs) is challenging because of its complex structure. Kidneys are derived from embryonic metanephros, which are composed of three progenitor cells: nephron progenitors, ureteric bud, and stromal progenitors. Nephron progenitors and ureteric bud have been induced successfully from PSCs as a result of the understanding of their detailed developmental process through cell-lineage tracing analysis. Moreover, these induced progenitors can be used to reconstruct the three-dimensional (3D) structure of kidneys in vitro, including glomeruli with podocytes, renal tubules, and the branching ureters. Induction of the remaining renal progenitors (that is, stromal progenitors from PSCs and the further maturation of reconstructed kidneys) needs to be studied extensively to regenerate functional and sophisticated kidneys from PSCs. In addition to the proper induction of renal progenitors, new bioengineering methods such as decellularization and 3D bioprinting and the recent advancements in the regeneration of kidneys in other species are promising leads for regenerating the complex spatial arrangement of kidneys, including the vascular network and urinary excretion pathway in humans.
KW - Kidney
KW - Pluripotent stem cells
KW - Renegeration
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U2 - 10.12688/f1000research.17127.1
DO - 10.12688/f1000research.17127.1
M3 - Review article
C2 - 30828435
AN - SCOPUS:85062390278
SN - 2046-1402
VL - 8
JO - F1000Research
JF - F1000Research
M1 - 216
ER -