Real-time multisite observation of glutamate release in rat hippocampal slices

Nahoko Kasai, Yasuhiko Jimbo, Osamu Niwa, Tomokazu Matsue, Keiichi Torimitsu

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

A multichannel glutamate sensor was fabricated that consists of enzyme modified electrodes and has a high sensitivity and selectivity to glutamate. We placed a rat hippocampal slice on the sensor and monitored the current at four electrodes resulting from the stimulation with muscimol, a γ-aminobutyric acidA (GABAA) receptor agonist. We obtained different glutamate concentration increases at the different positions, suppressed by bicuculline, a GABAA receptor antagonist. This demonstrated that the sensor can monitor the glutamate released via GABAA receptors pathways, and the difference in the concentrations may indicate differences in the distribution of GABAA receptor as well as diverse receptor functions. This multichannel sensor may be useful for non-invasive, real-time monitoring of glutamate distribution, which would make it a valuable tool for pharmacological analysis.

Original languageEnglish
Pages (from-to)112-116
Number of pages5
JournalNeuroscience Letters
Volume304
Issue number1-2
DOIs
Publication statusPublished - 2001 May 18

Keywords

  • Electrochemical detection
  • Electrode array
  • Glutamate
  • Glutamate oxidase
  • Horseradish peroxidase
  • γ-aminobutyric acid

ASJC Scopus subject areas

  • Neuroscience(all)

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