Real-time monitoring of L-glutamate release from mouse brain slices under ischemia with a glass capillary-based enzyme electrode

Naoto Nakamura, Kayoko Negishi, Ayumi Hirano, Masao Sugawara

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Real-time monitoring of L-glutamate release from various neuronal regions of mouse hippocampal slices under ischemia (a glucose-free hypoxia condition) is described. A glass capillary microelectrode with a tip size of 10 μm containing a very small volume (2 μL) of a solution of glutamate oxidase (GluOx) and ascorbate oxidase was used. First, the amperometric response behavior of the electrode at 0 V versus Ag/AgCl was characterized with a standard glutamate solution in terms of continuous measurements, effect of oxygen, viscosity of solution and concentration dependence. The electrode was applied to the real-time monitoring of L-glutamate released from different neuronal regions of acute hippocampal slices submerged in a hypoxia solution. The time-resolved amounts of L-glutamate released at various neuronal regions (CA1, CA3 and DG) of mouse hippocampal slices were quantified and compared with the reported L-glutamate fluxes using difference-image analysis during ischemia.

Original languageEnglish
Pages (from-to)660-667
Number of pages8
JournalAnalytical and Bioanalytical Chemistry
Volume383
Issue number4
DOIs
Publication statusPublished - 2005 Oct 1
Externally publishedYes

Keywords

  • Enzyme sensor
  • Glass capillary electrode
  • Ischemia
  • L-glutamate
  • Mouse hippocampal slices

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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