Real-time monitoring of extracellular l-glutamate levels released by high-frequency stimulation at region CA1 of hippocampal slices with a glass capillary-based l-glutamate sensor

Yuki Ikegami, Shizuko Hozumi, Atsushi Shoji, Ayumi Hirano-Iwata, Tim Bliss, Masao Sugawara

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Real-time monitoring of l-glutamate released by high-frequency stimulation in region CA1 of mouse hippocampal slices was performed with a glass capillary-based sensor, in combination with the recoding of excitatory postsynaptic potentials (fEPSPs). A method for extracting l-glutamate currents from the recorded ones was described and applied for determining the level of extracellular l-glutamate released by 100. Hz stimulation. Recording of an l-glutamate current with a current sampling interval of 1. Hz was found to be useful for acquiring a Faradaic current that reflects l-glutamate level released by the high-frequency stimulation of 7. trains, each 20. stimuli at 100. Hz and inter-train interval of 3. s. The l-glutamate level was obtained as 15. ±. 6. μM (n=8) for the persistent enhancement of fEPSPs, i.e., the induction of long-term potentiation (LTP), and 3. ±. 1. μM (n=5) for the case of no LTP induction. Based on these observations, the level of the extracellular l-glutamate was shown to play a crucial role in the induction of LTP.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalSensing and Bio-Sensing Research
Volume2
DOIs
Publication statusPublished - 2014 Dec 1

Keywords

  • Field excitatory postsynaptic potential
  • High-frequency stimulation
  • L-glutamate sensor
  • Long-term potentiation
  • Mouse hippocampal slice

ASJC Scopus subject areas

  • Biotechnology
  • Electronic, Optical and Magnetic Materials
  • Signal Processing
  • Electrical and Electronic Engineering

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