Real-time monitoring of cell death by surface infrared spectroscopy

Ryo Taro Yamaguchi; Ayumi Hirano-Iwata; Yasuo Kimura; Michio Niwano; Ko Ichiro Miyamoto; Hiroko Isoda; Hitoshi Miyazaki

doi:10.1063/1.2813013

Real-time monitoring of cell death by surface infrared spectroscopy

Ryo Taro Yamaguchi, Ayumi Hirano-Iwata, Yasuo Kimura, Michio Niwano, Ko Ichiro Miyamoto, Hiroko Isoda, Hitoshi Miyazaki

Research output: Contribution to journal › Article

11 Citations (Scopus)

Abstract

We have developed a method for real-time monitoring of the cell responses to cytotoxicants using Fourier transform infrared spectroscopy with the multiple internal reflection (MIR-FTIR) geometry. To prevent cell damages induced by measurement environments, we have constructed specialized chambers, in which temperature was maintained at (37±0.5) °C and humidified air containing 5% C O2 was supplied. We monitored cell death induced by cytotoxic surfactant Tween20 using MIR-FTIR spectroscopy. It was found that cell death can be monitored by the absorption intensity of amide II band. This result suggests that our method has a potential to be applied for real-time cytotoxicity assay.

Original language	English
Article number	203902
Journal	Applied Physics Letters
Volume	91
Issue number	20
DOIs	https://doi.org/10.1063/1.2813013
Publication status	Published - 2007

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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10.1063/1.2813013

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Yamaguchi, R. T., Hirano-Iwata, A., Kimura, Y., Niwano, M., Miyamoto, K. I., Isoda, H., & Miyazaki, H. (2007). Real-time monitoring of cell death by surface infrared spectroscopy. Applied Physics Letters, 91(20), [203902]. https://doi.org/10.1063/1.2813013

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AU - Yamaguchi, Ryo Taro

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AU - Miyamoto, Ko Ichiro

AU - Isoda, Hiroko

AU - Miyazaki, Hitoshi

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