Real-time monitoring of cell death by surface infrared spectroscopy

Ryo Taro Yamaguchi; Ayumi Hirano-Iwata; Yasuo Kimura; Michio Niwano; Ko Ichiro Miyamoto; Hiroko Isoda; Hitoshi Miyazaki

doi:10.1063/1.2813013

Real-time monitoring of cell death by surface infrared spectroscopy

Ryo Taro Yamaguchi, Ayumi Hirano-Iwata, Yasuo Kimura, Michio Niwano, Ko Ichiro Miyamoto, Hiroko Isoda, Hitoshi Miyazaki

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

We have developed a method for real-time monitoring of the cell responses to cytotoxicants using Fourier transform infrared spectroscopy with the multiple internal reflection (MIR-FTIR) geometry. To prevent cell damages induced by measurement environments, we have constructed specialized chambers, in which temperature was maintained at (37±0.5) °C and humidified air containing 5% C O2 was supplied. We monitored cell death induced by cytotoxic surfactant Tween20 using MIR-FTIR spectroscopy. It was found that cell death can be monitored by the absorption intensity of amide II band. This result suggests that our method has a potential to be applied for real-time cytotoxicity assay.

Original language	English
Article number	203902
Journal	Applied Physics Letters
Volume	91
Issue number	20
DOIs	https://doi.org/10.1063/1.2813013
Publication status	Published - 2007
Externally published	Yes

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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10.1063/1.2813013

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title = "Real-time monitoring of cell death by surface infrared spectroscopy",

abstract = "We have developed a method for real-time monitoring of the cell responses to cytotoxicants using Fourier transform infrared spectroscopy with the multiple internal reflection (MIR-FTIR) geometry. To prevent cell damages induced by measurement environments, we have constructed specialized chambers, in which temperature was maintained at (37±0.5) °C and humidified air containing 5% C O2 was supplied. We monitored cell death induced by cytotoxic surfactant Tween20 using MIR-FTIR spectroscopy. It was found that cell death can be monitored by the absorption intensity of amide II band. This result suggests that our method has a potential to be applied for real-time cytotoxicity assay.",

author = "Yamaguchi, {Ryo Taro} and Ayumi Hirano-Iwata and Yasuo Kimura and Michio Niwano and Miyamoto, {Ko Ichiro} and Hiroko Isoda and Hitoshi Miyazaki",

note = "Funding Information: This work was supported by a Grant-in-Aid for Basic Scientific Research (Grant Nos. 14205007 and 17206004) from the Ministry of Education, Science, Sports, and Culture of Japan.",

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doi = "10.1063/1.2813013",

language = "English",

volume = "91",

journal = "Applied Physics Letters",

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T1 - Real-time monitoring of cell death by surface infrared spectroscopy

AU - Yamaguchi, Ryo Taro

AU - Hirano-Iwata, Ayumi

AU - Kimura, Yasuo

AU - Niwano, Michio

AU - Miyamoto, Ko Ichiro

AU - Isoda, Hiroko

AU - Miyazaki, Hitoshi

N1 - Funding Information: This work was supported by a Grant-in-Aid for Basic Scientific Research (Grant Nos. 14205007 and 17206004) from the Ministry of Education, Science, Sports, and Culture of Japan.

PY - 2007

Y1 - 2007

N2 - We have developed a method for real-time monitoring of the cell responses to cytotoxicants using Fourier transform infrared spectroscopy with the multiple internal reflection (MIR-FTIR) geometry. To prevent cell damages induced by measurement environments, we have constructed specialized chambers, in which temperature was maintained at (37±0.5) °C and humidified air containing 5% C O2 was supplied. We monitored cell death induced by cytotoxic surfactant Tween20 using MIR-FTIR spectroscopy. It was found that cell death can be monitored by the absorption intensity of amide II band. This result suggests that our method has a potential to be applied for real-time cytotoxicity assay.

AB - We have developed a method for real-time monitoring of the cell responses to cytotoxicants using Fourier transform infrared spectroscopy with the multiple internal reflection (MIR-FTIR) geometry. To prevent cell damages induced by measurement environments, we have constructed specialized chambers, in which temperature was maintained at (37±0.5) °C and humidified air containing 5% C O2 was supplied. We monitored cell death induced by cytotoxic surfactant Tween20 using MIR-FTIR spectroscopy. It was found that cell death can be monitored by the absorption intensity of amide II band. This result suggests that our method has a potential to be applied for real-time cytotoxicity assay.

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Real-time monitoring of cell death by surface infrared spectroscopy

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