Real-time monitoring of cell death by surface infrared spectroscopy

Ryo Taro Yamaguchi, Ayumi Hirano-Iwata, Yasuo Kimura, Michio Niwano, Ko Ichiro Miyamoto, Hiroko Isoda, Hitoshi Miyazaki

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have developed a method for real-time monitoring of the cell responses to cytotoxicants using Fourier transform infrared spectroscopy with the multiple internal reflection (MIR-FTIR) geometry. To prevent cell damages induced by measurement environments, we have constructed specialized chambers, in which temperature was maintained at (37±0.5) °C and humidified air containing 5% C O2 was supplied. We monitored cell death induced by cytotoxic surfactant Tween20 using MIR-FTIR spectroscopy. It was found that cell death can be monitored by the absorption intensity of amide II band. This result suggests that our method has a potential to be applied for real-time cytotoxicity assay.

Original languageEnglish
Article number203902
JournalApplied Physics Letters
Volume91
Issue number20
DOIs
Publication statusPublished - 2007

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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