Abstract
The direct observation of individual molecules in action is required for a better understanding of the mechanisms of biological reactions. We used a high-speed atomic force microscope (AFM) in solution to visualize short DNA fragments in motion. The technique represents a new approach in analyzing molecular interactions, and it allowed us to observe real-time images of biotinylated DNA binding to/dissociating from streptavidin protein. Our results show that high-speed AFMs have the potential to reveal the mechanisms of molecular interactions, which cannot be determined by analyzing the average value of mass reactions.
Original language | English |
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Pages (from-to) | 184-190 |
Number of pages | 7 |
Journal | Ultramicroscopy |
Volume | 107 |
Issue number | 2-3 |
DOIs | |
Publication status | Published - 2007 Feb |
Externally published | Yes |
Keywords
- AFM
- DNA
- Dynamics
- Protein
- Real-time imaging
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Instrumentation