Real-time imaging of DNA-streptavidin complex formation in solution using a high-speed atomic force microscope

Mime Kobayashi, Koji Sumitomo, Keiichi Torimitsu

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

The direct observation of individual molecules in action is required for a better understanding of the mechanisms of biological reactions. We used a high-speed atomic force microscope (AFM) in solution to visualize short DNA fragments in motion. The technique represents a new approach in analyzing molecular interactions, and it allowed us to observe real-time images of biotinylated DNA binding to/dissociating from streptavidin protein. Our results show that high-speed AFMs have the potential to reveal the mechanisms of molecular interactions, which cannot be determined by analyzing the average value of mass reactions.

Original languageEnglish
Pages (from-to)184-190
Number of pages7
JournalUltramicroscopy
Volume107
Issue number2-3
DOIs
Publication statusPublished - 2007 Feb 1
Externally publishedYes

Keywords

  • AFM
  • DNA
  • Dynamics
  • Protein
  • Real-time imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Instrumentation

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