Abstract
We developed a real-time electrochemical imaging method that uses a multiple enzyme-modified microelectrode. The method will enable the investigation of the functions of biological materials and cells. To test its effectiveness, we imaged the two-dimensional concentration distribution for hydrogen peroxide and L-glutamate in a standard solution. The multiple electrode consists of an 8 x 8 array of 30 x 30 μm2 carbon micro electrode. Each electrode was connected to a 64-channel potentiostat that could apply a potential to all electrodes at the same time. The multiple electrode was coated with an Os-polyvinylpyridine based polymer (Os-gel) containing horse radish peroxidase (HRP) to detect hydrogen peroxide, which is a very common product of oxidase enzyme. When measuring glutamate, which is a well-known neurotransmitter in the mammalian central nerve system, we modified the electrode with a bilayer of Os-gel-HRP and GluOx. The detection limit of our method was 1 μM and images of the glutamate concentration-distribution changes induced by local injection of glutamate through microcapillary were obtained in real time. (C) 2000 Elsevier Science S.A.
Original language | English |
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Pages (from-to) | 523-529 |
Number of pages | 7 |
Journal | Biosensors and Bioelectronics |
Volume | 15 |
Issue number | 9-10 |
DOIs | |
Publication status | Published - 2000 Nov 1 |
Externally published | Yes |
Keywords
- Concentration distribution
- Electrochemical imaging
- Enzyme-modified electrode
- Multiple electrode
- Real-time
ASJC Scopus subject areas
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry