Real-time electrochemical imaging using an individually addressable multi-channel electrode

Katsuyoshi Hayashi, Tsutomu Horiuchi, Ryoji Kurita, Keiichi Torimitsu, Osamu Niwa

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

We developed a real-time electrochemical imaging method that uses a multiple enzyme-modified microelectrode. The method will enable the investigation of the functions of biological materials and cells. To test its effectiveness, we imaged the two-dimensional concentration distribution for hydrogen peroxide and L-glutamate in a standard solution. The multiple electrode consists of an 8 x 8 array of 30 x 30 μm2 carbon micro electrode. Each electrode was connected to a 64-channel potentiostat that could apply a potential to all electrodes at the same time. The multiple electrode was coated with an Os-polyvinylpyridine based polymer (Os-gel) containing horse radish peroxidase (HRP) to detect hydrogen peroxide, which is a very common product of oxidase enzyme. When measuring glutamate, which is a well-known neurotransmitter in the mammalian central nerve system, we modified the electrode with a bilayer of Os-gel-HRP and GluOx. The detection limit of our method was 1 μM and images of the glutamate concentration-distribution changes induced by local injection of glutamate through microcapillary were obtained in real time. (C) 2000 Elsevier Science S.A.

Original languageEnglish
Pages (from-to)523-529
Number of pages7
JournalBiosensors and Bioelectronics
Volume15
Issue number9-10
DOIs
Publication statusPublished - 2000 Nov 1
Externally publishedYes

Keywords

  • Concentration distribution
  • Electrochemical imaging
  • Enzyme-modified electrode
  • Multiple electrode
  • Real-time

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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