RCAN1-4 knockdown attenuates cell growth through the inhibition of Ras signaling

Hong Joon Lee, Young Sun Kim, Yasufumi Sato, Young Jin Cho

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Forced changes in the expression of regulator of calcineurin 1 (RCAN1) affects cell growth. This has been linked to the suppression of calcineurin-nuclear factor of activated T cells signaling by RCAN1. Here, we describe a novel role of RCAN1 isoform 4 in proper expression of Ras protein and its signaling. RCAN1 isoform 4 knockdown attenuated growth factor-induced extracellular signal-regulated kinase activation and cell growth; reduced Ras levels and its translation rate; and led to a reduction of eukaryotic initiation factor 4E in the initiation complex and a slight repression of global protein synthesis. Experiments utilizing activity-modified mutants of calcineurin A demonstrated that these effects were calcineurin-independent. Our findings reveal a previously unknown role of RCAN1-4 in protein synthesis, which may be relevant to cell growth.

Original languageEnglish
Pages (from-to)2557-2564
Number of pages8
JournalFEBS Letters
Volume583
Issue number15
DOIs
Publication statusPublished - 2009 Aug 6

Keywords

  • Cell growth
  • ERK/MAPK
  • RCAN1/DSCR1
  • Ras
  • Translation
  • eIF4E

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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