Ratiometric imaging of intracellular Mg2+ dynamics using a red fluorescent turn-off probe and a green fluorescent turn-on probe

Yusuke Matsui, Shin Mizukami, Kazuya Kikuchi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

In this work, we developed a red fluorescent turn-off Mg2+ probe with high Mg2+ selectivity. The quenching response to Mg2+ in combination with a green fluorescent turn-on Mg2+ probe enabled a ratiometric imaging of the changes in intracellular Mg2+ concentration. This ratiometric imaging system significantly enhanced the detection sensitivity of intracellular Mg2+ fluctuations in comparison with a conventional ratiometric Mg2+ sensing system, thus leading to a valuable technique for visualizing small change in intracellular Mg2+ concentration.

Original languageEnglish
Pages (from-to)23-26
Number of pages4
JournalChemistry Letters
Volume47
Issue number1
DOIs
Publication statusPublished - 2018

Keywords

  • Intracellular Mg concentration
  • Ratiometric imaging
  • Red fluorescent turn-off probe

ASJC Scopus subject areas

  • Chemistry(all)

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