Ratiometric fluorescent signaling of small molecule, environmentally sensitive dye conjugates for detecting single-base mutations in DNA

Chun Xia Wang, Yusuke Sato, Megumi Kudo, Seiichi Nishizawa, Norio Teramae

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Bind it! A ratiometric fluorescent probe based on conjugation of a DNA-binding ligand with an environmentally sensitive dye to probe hydrophobic grooves of the DNA helix was developed. The resulting conjugate ATMND-DBD can bind to an orphan nucleobase opposite an abasic (AP) site with high selectivity and affinity for pyrimidine bases (see figure).

Original languageEnglish
Pages (from-to)9481-9484
Number of pages4
JournalChemistry - A European Journal
Volume18
Issue number31
DOIs
Publication statusPublished - 2012 Jul 27

Keywords

  • DNA recognition
  • fluorescent probes
  • ligand design
  • noncovalent interactions
  • nucleobases

ASJC Scopus subject areas

  • Catalysis
  • Organic Chemistry

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