Ratiometric fluorescence detection of pyrimidine/purine transversion by using a 2-amino-1,8-naphthyridine derivative

Hiroyuki Satake, Seiichi Nishizawa, Norio Teramae

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

A new class of abasic site-binding fluorescence ligands, Naph-NBD in which 7-nitrobenzo-2-oxa-1,3-diazole (NBD) is connected to 2-amino-7-methyl-1,8- naphthyridine (Naph) by a propylene linker, is presented for the ratiometric assay for SNPs typing. In solutions buffered to pH 7.0 (I = 0.11 M, at 5°C), Naph-NBD is found to selectively recognize pyrimidine bases over purine bases opposite the abasic site in DNA duplexes (K 11 /M 1 : T, 8.1 x 10 6 ; C, 2.5 x 10 6 ; G, 0.33 x 10 6 ; A, 0.27 x 10 6 ). The binding of Naph-NBD is accompanied by significant quenching of the fluorescence from the naphthyridine moiety (λ max ;, 409 nm), while the emission from the NBD (λ max ;, 544 nm) is relatively unaffected. Such a fluorescence response of Naph-NBD allows the emission ratio detection of pyrimidine/purine transversion. 2006

Original languageEnglish
Pages (from-to)195-197
Number of pages3
Journalanalytical sciences
Volume22
Issue number2
DOIs
Publication statusPublished - 2006 Feb

ASJC Scopus subject areas

  • Analytical Chemistry

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