Rat mesangial cells actively produce phoshatidylinositol-anchored Thy-1

T. Miyata, K. Isobe, R. Inagi, R. Taguchi, H. Ikezawa, I. Takai, Y. Fujita, T. Iwamoto, T. Hasegawa, O. Oda, N. Yamanaka, S. Sugiyama, K. Maeda, K. Yamada, I. Nakashima

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Abstract

We provide evidence that the mesangial cells of rat kidney glomeruli express Thy-1 as a phosphatidylinositol-anchored protein. Both the mesangial area of kidney, examined in tissue sections, and mesangial cells maintained in culture for more than 3 months, showed prominent immunofluorescence staining with an anti-Thy-1 monoclonal antibody (OX7); this staining was almost completely abolished by pretreating kidney sections or mesangial cells with the phosphatidylinositol-specific enzyme, phospholipase C. By Northern blotting, mesangial cells were shown to express mRNA of an appropriate size, hybridizing to a mouse Thy-1.1-specific probe.

Original languageEnglish
Pages (from-to)531-533
Number of pages3
JournalImmunology
Volume67
Issue number4
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Miyata, T., Isobe, K., Inagi, R., Taguchi, R., Ikezawa, H., Takai, I., Fujita, Y., Iwamoto, T., Hasegawa, T., Oda, O., Yamanaka, N., Sugiyama, S., Maeda, K., Yamada, K., & Nakashima, I. (1989). Rat mesangial cells actively produce phoshatidylinositol-anchored Thy-1. Immunology, 67(4), 531-533.