Rat kidney thromboxane synthase: cDNA cloning and gene expression regulation in hydronephrotic kidney

Eikatsu Tsutsumi, Kazuhisa Takeuchi, Takaaki Abe, Nobuyuki Takahashi, Taro Kato, Yoshihiro Taniyama, Yukio Ikeda, Sadayoshi Ito, Keishi Abe

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

We isolated a rat homolog of thromboxane (TX) synthase cDNA (~1.8 kb) from the kidney with a fragment of human TX synthase cDNA amplified by polymerase chain reaction with placenta cDNA as a template. Northern blot analysis has shown that rat TX synthase gene is expressed abundantly in lung, liver, and uterus; moderately in kidney. TX synthase mRNA expression was up- regulated in hydronephrotic kidney made by ureter ligation. In conclusion, we have revealed the structure of rat kidney TX synthase. Up-regulation of renal TX synthase, which may cause stimulation of TX synthesis, is possibly implicated in the tissue injury in hydronephrotic kidney.

Original languageEnglish
Pages (from-to)423-431
Number of pages9
JournalProstaglandins
Volume53
Issue number6
DOIs
Publication statusPublished - 1997 Jun

Keywords

  • Gene expression
  • Hydronephrosis
  • Prostaglandin

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Fingerprint Dive into the research topics of 'Rat kidney thromboxane synthase: cDNA cloning and gene expression regulation in hydronephrotic kidney'. Together they form a unique fingerprint.

Cite this