Rat gastric prepepsinogen: In Vitro synthesis and partial amino-terminal signal sequence

Yoshikazu Ichihara, Kazuhiro Sogawa, Kenji Takahashi

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    The major translation product of rat gastric mucosa RNA in a wheat germ cell-free system was identified as prepepsinogen by electrophoretic analysis of its immuno-precipitate on sodium dodecyl sulfate (SDS)-polyacrylamide gels and amino-terminal sequence determination. The translation product containing radioactive amino acids, purified by SDS-polyacrylamide gel electrophoresis, was shown to have an amino-terminal extension peptide comprising 16 amino acid residues. A partial amino acid sequence of this extension peptide is as follows: Met-X-X-Met-Val-Val-X-Leu-Leu-X-Leu-X-Leu-Leu-X-X-pepsinogen.

    Original languageEnglish
    Pages (from-to)603-606
    Number of pages4
    JournalJournal of biochemistry
    Volume92
    Issue number2
    DOIs
    Publication statusPublished - 1982 Jan 1

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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