Ras-mediated signaling pathway regulates the expression of a low-molecular-weight heat-shock protein in fission yeast

Inaho Danjoh, Asao Fujiyama

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

In fission yeast, Schizosaccharomyces pombe, deficiency of ras1 gene causes an abnormal cell shape and abolishes mating ability. However, target genes of this signaling pathway are largely unknown because of the lack of an appropriate analysis system. To overcome this problem, we have started a novel project to categorize entire genes based on their expression levels under different growth conditions. Using this strategy, we screened genes whose expression levels were affected in the presence or absence of the ras1 gene product. For this purpose, we utilized high-density arrays of clones covering the entire genome of the fission yeast, and probed with labelled cDNA derived from various strains and growth conditions. Here, we demonstrate the detection of a low-molecular-weight heat-shock protein gene, hsp16, whose expression is very likely to be regulated by a ras-mediated signaling pathway, but not by the heat-shock response. (C) 1999 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)347-352
Number of pages6
JournalGene
Volume236
Issue number2
DOIs
Publication statusPublished - 1999 Aug 20
Externally publishedYes

Keywords

  • Differential display
  • Functional genomics
  • Genome
  • Reverse Northern

ASJC Scopus subject areas

  • Genetics

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