Rapid turnover of low density lipoprotein receptor in human monocytic THP-1 cells

Ryoji Hamanaka, Tadashi Seguchi, Yasufumi Sato, Mayumi Ono, Kimitoshi Kohno, Michihiko Kuwano

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

We examined whether human monocyte-derived macrophages had low density lipoprotein (LDL) receptors with a short life span. The human monocytic leukemia cell line, THP-1, was highly differentiated when treated with phorbol ester. LDL receptors degraded rapidly with half-lives of 3-4 h in THP-1 cells before phorbol ester treatment. During the transition into monocytic cells, expression of the LDL receptor gene was not affected. However, relative degradation rates of LDL receptors normalized by those of cellular total proteins were about twice as fast in phorbol ester-treated THP-1 cells compared to untreated cells.

Original languageEnglish
Pages (from-to)261-263
Number of pages3
JournalFEBS Letters
Volume294
Issue number3
DOIs
Publication statusPublished - 1991 Dec 9
Externally publishedYes

Keywords

  • Human monocytic leukemia THP-1 cell
  • Low density lipoprotein receptor

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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