Rapid detection of S-glycoproteins of self-incompatible crucifers using Con-A reaction

Takeshi Nishio, Kokichi Hinata

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

As S-specific glycoproteins react with concanavalin A (ConA), they could be stained sensitively with ConA-FITC after cellulose acetate isoelectric focusing of stigma proteins in self-incompatible crucifers. By this method the S genotype of an individual plant could be inferred within 4 h using 4 flowers. This simple and rapid method may be applicable for describing S genotypes in practical F1 breeding.

Original languageEnglish
Pages (from-to)217-221
Number of pages5
JournalEuphytica
Volume29
Issue number2
DOIs
Publication statusPublished - 1980 Jun 1

Keywords

  • Brassica campestris
  • Brassica oleracea
  • Con-A reaction
  • Raphanus sativus
  • S-specific glycoprotein
  • self-incompatibility

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture

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