Rapid detection of homologously integrated DNA fragments and accurate quantitation of their copy number in transgenic Aspergillus oryzae by PCR

Munechika Honda, Toshitaka Minetoki, Katsuya Gomi, Masayuki Machida

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A simple and rapid method for the analysis of artificially introduced DNA fragments has been developed using competitive PCR and long and accurate PCR. The locus and the copy number of the DNA fragments in each Aspergillus oryzae transformant could be detected more rapidly and accurately by this method than by the conventional Southern hybridization method.

Original languageEnglish
Pages (from-to)577-579
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume90
Issue number5
DOIs
Publication statusPublished - 2000 Jan 1

Keywords

  • Aspergillus oryzae
  • Competitive PCR
  • Gene copy number
  • LA-PCR

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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