Rapid detection of homologously integrated DNA fragments and accurate quantitation of their copy number in transgenic Aspergillus oryzae by PCR

Munechika Honda, Toshitaka Minetoki, Katsuya Gomi, Masayuki Machida

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    A simple and rapid method for the analysis of artificially introduced DNA fragments has been developed using competitive PCR and long and accurate PCR. The locus and the copy number of the DNA fragments in each Aspergillus oryzae transformant could be detected more rapidly and accurately by this method than by the conventional Southern hybridization method.

    Original languageEnglish
    Pages (from-to)577-579
    Number of pages3
    JournalJournal of Bioscience and Bioengineering
    Volume90
    Issue number5
    DOIs
    Publication statusPublished - 2000 Jan 1

    Keywords

    • Aspergillus oryzae
    • Competitive PCR
    • Gene copy number
    • LA-PCR

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

    Fingerprint

    Dive into the research topics of 'Rapid detection of homologously integrated DNA fragments and accurate quantitation of their copy number in transgenic Aspergillus oryzae by PCR'. Together they form a unique fingerprint.

    Cite this