Rapid detection of homologously integrated DNA fragments and accurate quantitation of their copy number in transgenic Aspergillus oryzae by PCR

Munechika Honda; Toshitaka Minetoki; Katsuya Gomi; Masayuki Machida

doi:10.1016/S1389-1723(01)80046-3

Rapid detection of homologously integrated DNA fragments and accurate quantitation of their copy number in transgenic Aspergillus oryzae by PCR

Munechika Honda, Toshitaka Minetoki, Katsuya Gomi, Masayuki Machida

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A simple and rapid method for the analysis of artificially introduced DNA fragments has been developed using competitive PCR and long and accurate PCR. The locus and the copy number of the DNA fragments in each Aspergillus oryzae transformant could be detected more rapidly and accurately by this method than by the conventional Southern hybridization method.

Original language	English
Pages (from-to)	577-579
Number of pages	3
Journal	Journal of Bioscience and Bioengineering
Volume	90
Issue number	5
DOIs	https://doi.org/10.1016/S1389-1723(01)80046-3
Publication status	Published - 2000 Jan 1

Keywords

Aspergillus oryzae
Competitive PCR
Gene copy number
LA-PCR

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/S1389-1723(01)80046-3

Cite this

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abstract = "A simple and rapid method for the analysis of artificially introduced DNA fragments has been developed using competitive PCR and long and accurate PCR. The locus and the copy number of the DNA fragments in each Aspergillus oryzae transformant could be detected more rapidly and accurately by this method than by the conventional Southern hybridization method.",

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AU - Honda, Munechika

AU - Minetoki, Toshitaka

AU - Gomi, Katsuya

AU - Machida, Masayuki

PY - 2000/1/1

Y1 - 2000/1/1

N2 - A simple and rapid method for the analysis of artificially introduced DNA fragments has been developed using competitive PCR and long and accurate PCR. The locus and the copy number of the DNA fragments in each Aspergillus oryzae transformant could be detected more rapidly and accurately by this method than by the conventional Southern hybridization method.

AB - A simple and rapid method for the analysis of artificially introduced DNA fragments has been developed using competitive PCR and long and accurate PCR. The locus and the copy number of the DNA fragments in each Aspergillus oryzae transformant could be detected more rapidly and accurately by this method than by the conventional Southern hybridization method.

KW - Aspergillus oryzae

KW - Competitive PCR

KW - Gene copy number

KW - LA-PCR

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SN - 1389-1723

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ER -

Rapid detection of homologously integrated DNA fragments and accurate quantitation of their copy number in transgenic Aspergillus oryzae by PCR

Abstract

Keywords

ASJC Scopus subject areas

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