A simple and rapid method for the analysis of artificially introduced DNA fragments has been developed using competitive PCR and long and accurate PCR. The locus and the copy number of the DNA fragments in each Aspergillus oryzae transformant could be detected more rapidly and accurately by this method than by the conventional Southern hybridization method.
- Aspergillus oryzae
- Competitive PCR
- Gene copy number
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology