Rapid biochemical synthesis of 11C-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis

Takayoshi Matsuda, Shozo Furumoto, Kae Higuchi, Jun Yokoyama, Ming Rong Zhang, Kazuhiko Yanai, Ren Iwata, Takanori Kigawa

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Immuno-PET is a promising approach for improved cancer diagnosis, by taking advantage of the high specificity of antibodies. Here, we present a novel cell-free protein synthesis method for preparing a positron emitter labeled-antibody. Functional anti-human EGFRvIII single chain Fv, MR1-1, was successfully labeled with carbon-11 (half-life = 20.4 min) in 5 min (36% yield) by the direct incorporation of the clinical PET tracer, l-[11C] methionine. The product [11C]MR1-1 was easily and rapidly isolated with high radiochemical purity (>95%) from the reaction solution, by affinity purification. This method would be widely applicable to the preparation of radiolabeled antibodies for PET imaging.

Original languageEnglish
Pages (from-to)6579-6582
Number of pages4
JournalBioorganic and Medicinal Chemistry
Volume20
Issue number22
DOIs
Publication statusPublished - 2012 Nov 15

Keywords

  • Cell-free protein synthesis
  • EGFR
  • PET imaging
  • [C] Methionine
  • scFv

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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