Rapid and specific screening assay for kras oncogene mutation by a novel gene amplification method

Akira Kumasaka, Naoyuki Matsumoto, Shotaro Mukae, Taiichi Kitano, Hiroyasu Noguchi, Hidero Ohki, Kazuo Komiyama, Tomohiro Ando

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Background: Epidermal growth factor receptor (EGFR) is a target of molecular therapeutics for colorectal cancer. However, mutation of Kirsten rat sarcoma viral oncogene homolog (KRAS) gene at codons 12 and 13 attenuates the therapeutic effect of anti-EGFR therapies. Therefore, the detection of KRAS gene mutation is important for therapeutic decision-making. Materials and Methods: KRAS gene mutation at codons 12 (c.34G>T, c.35G>C, c.35G>A) and 13 (c.38G>A) in six cancer cell lines were investigated. A loop-mediated isothermal amplification-based procedure was developed that employed peptide nucleic acid to suppress amplification of the wild-type allele. Results: This mutation-oriented gene-amplification procedure can amplify the DNA fragment of the KRAS gene with codon 12 and codon 13 mutation within 30 min. Moreover, boiled cells can work as template resources. Conclusion: This newly developed procedure can be useful for patient stratification for anti-EGFR therapies.

Original languageEnglish
Pages (from-to)1571-1579
Number of pages9
JournalAnticancer research
Issue number4
Publication statusPublished - 2016 Apr
Externally publishedYes


  • KRAS mutation
  • LAMP
  • Molecular targeted therapy
  • Stratification biomarker

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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