Rapid and simple immunoassay based on negative dielectrophoresis with three-dimensional interdigitated array electrodes

T. Yasukawa, Hitoshi Shiku, Tomokazu Matsue, F. Mizutani

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Rapid accumulations of particles and living cells with negative dielectrophoresis (n-DEP) have been applied to develop the rapid and simple immunosensing method. Grid formation of electrodes was fabricated by rotating the upper template interdigitated microband array (IDA) electrode by 90° relative to the lower IDA. When AC electric signal was applied to the bands on the upper and lower IDA, island organization was rapidly formed at the intersections with low electric fields. The accumulated particles were fixed through the immunoreactions between the antibody immobilized on the particle surface and analytes in the solution. The presence of the specific antigens allowed the formation of fixed complexes of particles. It is noted that the time required for single sensing is as short as 5 min and separation steps are eliminated in the presented procedure. We demonstrated the rapid and simple immunosensing using the aggregation of particles accumulated with DEP.

Original languageEnglish
Title of host publicationChemical Sensors 10 -and- MEMS/NEMS 10
Pages139-146
Number of pages8
Edition12
DOIs
Publication statusPublished - 2012 Dec 1
EventSymposia on Chemical Sensors 10 - Chemical and Biological Sensors and Analytical Systems and Microfabricated and Nanofabricated Systems for MEMS/NEMS 10 - 222nd ECS Meeting/PRiME 2012 - Honolulu, HI, United States
Duration: 2012 Oct 72012 Oct 12

Publication series

NameECS Transactions
Number12
Volume50
ISSN (Print)1938-5862
ISSN (Electronic)1938-6737

Other

OtherSymposia on Chemical Sensors 10 - Chemical and Biological Sensors and Analytical Systems and Microfabricated and Nanofabricated Systems for MEMS/NEMS 10 - 222nd ECS Meeting/PRiME 2012
Country/TerritoryUnited States
CityHonolulu, HI
Period12/10/712/10/12

ASJC Scopus subject areas

  • Engineering(all)

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