Rapid and simple detection of isoniazid-resistant mycobacterium tuberculosis utilizing a dna chromatography-based technique

Takuya Kodera, Tomoyuki Yamaguchi, Yukari Fukushima, Kumi Kobayashi, Yutaka Takarada, Joseph Yamweka Chizimu, Chie Nakajima, Eddie Samuneti Solo, Patrick Saili Lungu, Mitsuo Kawase, Yasuhiko Suzuki

Research output: Contribution to journalArticlepeer-review

Abstract

Despite the availability of anti-tuberculosis drugs, the treatment of tuberculosis has been complicated by drug-resistant tuberculosis. The early detection of drug resistance makes early treatment possible. However, the available tools are mainly for rifampicin resistance detection, and the existing isoniazid resistance detection method is expensive, highly technical, and complicated, making it unsustainable for use in developing nations. This study aimed to develop a simple, rapid, and low-cost diagnostic kit for isoniazid-resistant tuberculosis using the single-stranded tag hybridization method to target an isoniazid resistance-conferring mutation. Specificity and sensitivity were assessed using DNA extracted from 49 isoniazid-resistant and 41 isoniazid-susceptible Mycobacterium tuberculosis clinical isolates cultured in mycobacterial growth indicator tubes. Positive signals were observed on mutant and wild-type lines with 100% sensitivity and specificity compared with Sanger sequencing results. In contrast, no positive signal was observed for non-tuberculosis mycobacteria. The detection limit of this method was 103 CFU or less. The STH-PAS system for isoniazid-resistant M. tuberculosis detection developed in this study offers a better alternative to conventional phenotypic isoniazid resistance determination, which will be of both clinical and epidemiological significance in resource-limited nations.

Original languageEnglish
Pages (from-to)214-219
Number of pages6
JournalJapanese journal of infectious diseases
Volume74
Issue number3
DOIs
Publication statusPublished - 2021
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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