Rapid and high-throughput formation of 3D embryoid bodies in hydrogels using the dielectrophoresis technique

Samad Ahadian, Shukuyo Yamada, Javier Ramón-Azcón, Kosuke Ino, Hitoshi Shiku, Ali Khademhosseini, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

In this manuscript, we demonstrate the rapid formation of three-dimensional (3D) embryonic stem cell (ESC) aggregates with controllable sizes and shapes in hydrogels using dielectrophoresis (DEP). The ESCs encapsulated within a methacrylated gelatin (GelMA) prepolymer were introduced into a DEP device and, upon applying an electric field and crosslinking of the GelMA hydrogel, formed 3D ESC aggregates. Embryoid bodies (EBs) fabricated using this method showed high cellular viability and pluripotency. The proposed technique enables production of EBs on a large scale and in a high-throughput manner for potential cell therapy and tissue regeneration applications.

Original languageEnglish
Pages (from-to)3690-3694
Number of pages5
JournalLab on a Chip
Volume14
Issue number19
DOIs
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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