Random BAC FISH of monocot plants reveals differential distribution of repetitive DNA elements in small and large chromosome species

Go Suzuki, Yuka Ogaki, Nozomi Hokimoto, Lin Xiao, Akie Kikuchi-Taura, Chiaki Harada, Ryozo Okayama, Asami Tsuru, Misa Onishi, Naoko Saito, Geum Sook Do, Sun Hee Lee, Takuro Ito, Akira Kanno, Maki Yamamoto, Yasuhiko Mukai

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    BAC FISH (fluorescence in situ hybridization using bacterial artificial chromosome probes) is a useful cytogenetic technique for physical mapping, chromosome marker screening, and comparative genomics. As a large genomic fragment with repetitive sequences is inserted in each BAC clone, random BAC FISH without adding competitive DNA can unveil complex chromosome organization of the repetitive elements in plants. Here we performed the comparative analysis of the random BAC FISH in monocot plants including species having small chromosomes (rice and asparagus) and those having large chromosomes (hexaploid wheat, onion, and spider lily) in order to understand a whole view of the repetitive element organization in Poales and Asparagales monocots. More unique and less dense dispersed signals of BAC FISH were observed in species with smaller chromosomes in both the Poales and Asparagales species. In the case of large-chromosome species, 75-85% of the BAC clones were detected as dispersed repetitive FISH signals along entire chromosomes. The BAC FISH of Lycoris did not even show localized repetitive patterns (e.g., centromeric localization) of signals.

    Original languageEnglish
    Pages (from-to)621-628
    Number of pages8
    JournalPlant Cell Reports
    Volume31
    Issue number4
    DOIs
    Publication statusPublished - 2012 Apr

    Keywords

    • Bacterial artificial chromosome (BAC)
    • Chromosome
    • Fluorescence in situ hybridization (FISH)
    • Monocot
    • Repetitive element

    ASJC Scopus subject areas

    • Agronomy and Crop Science
    • Plant Science

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