Random amplified polymorphic DNA (RAPD) variation among populations of the insular endemic plant Campanula microdonta (Campanulaceae)

S. Oiki, T. Kawahara, K. Inoue, M. Ohara, M. Maki

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

RAPD variation was examined in nine populations of Campanula microdonta Koidz., endemic to the Izu Islands, Japan. Ninety-eight bands were obtained for all populations, 94 % of which were polymorphic at least within a population. Shannon's H values were calculated; these have frequently been used in RAPD studies to estimate genetic diversity. The values within populations did not correlate with the allozyme gene diversity estimated by a previous study or with distance from the Japanese mainland. The possible reasons for this discrepancy are different selection regimes between the two markers, higher RAPD mutation rates, and each marker's different coverage of genomes. Cluster analysis of genetic similarities suggested that colonization of each island probably occurred once, except for Miyake Island, where immigration has occurred at least twice.

Original languageEnglish
Pages (from-to)661-667
Number of pages7
JournalAnnals of botany
Volume87
Issue number5
DOIs
Publication statusPublished - 2001 Jan 1

Keywords

  • AMOVA
  • Campanula microdonta Koidz.
  • Genetic diversity
  • Insular endemic plant
  • Population genetic structure
  • RAPD

ASJC Scopus subject areas

  • Plant Science

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