Raman spectroscopic study on the conformation of a peptide fragment representing the DNA-binding domain of filamentous virus Pf3 coat protein

Takashi Miura, Hideo Takeuchi, Issei Harada

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Raman spectra have been measured of a nonapeptide which has an amino acid sequence identical to that of the C-terminal region of the major coat protein subunit of Filamentous bacteriophage Pf3. The peptide shows a strong tendency to form a β-sheet structure in aqueous solution. The β-sheet formation is significantly promoted by complexation with single-stranded DNA but not with double-stranded DNA. It is suggested that the C-terminal region of the Pf3 coat protein binds to the single-stranded DNA genome in the virion with a β-sheet conformation, in sharp contrast with the α-helical binding in other filamentous bacteriophages.

Original languageEnglish
Pages (from-to)181-184
Number of pages4
JournalFEBS Letters
Volume307
Issue number2
DOIs
Publication statusPublished - 1992 Jul 28

Keywords

  • Coat protein
  • DNA-protein interaction
  • Filamentous virus
  • Raman spectroscopy
  • Secondary structure

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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