Raman imaging microscopy for quantitative analysis of biological samples

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Raman imaging microscopy is a powerful tool for label-free imaging of biological samples. It has the advantage of measuring the spatial distribution of endogenous proteins and lipids in cells, as well as obtaining chemical information on these endogenous molecules, such as hydrogen bonding and electrostatic interactions. However, because Raman intensity is very weak compared with fluorescence intensity, obtaining a reliable Raman image requires fast acquisition of a Raman image and rejection of background fluorescence. In this chapter, we describe the procedure for obtaining images of the Raman band of interest using a multipoint technique, which is the fast acquisition method for obtaining an image.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages163-172
Number of pages10
Volume1035
DOIs
Publication statusPublished - 2017

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1035
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Keywords

  • Cell medium
  • Label-free imaging
  • Molecular vibrations
  • Multipoint microscopy
  • Raman imaging microscopy
  • Raman spectrum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Kajimoto, S., Takeuchi, M., & Nakabayashi, T. (2017). Raman imaging microscopy for quantitative analysis of biological samples. In Advances in Experimental Medicine and Biology (Vol. 1035, pp. 163-172). (Advances in Experimental Medicine and Biology; Vol. 1035). Springer New York LLC. https://doi.org/10.1007/978-3-319-67358-5_12