Raman cell imaging with boron cluster molecules conjugated with biomolecules

Masahito Mochizuki, Shinichi Sato, Syifa Asatyas, Zbigniew J. Leśnikowski, Tomohiro Hayashi, Hiroyuki Nakamura

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Raman spectroscopic measurements and theoretical calculation revealed that the Raman bands corresponding to the B-H stretching vibrations of two types of simple icosahedral boron clusters, ortho-carborane 3 and closo-dodecaborate 4 appeared at approximately 2450-2700 cm-1, and did not overlap with those of cellular components. Although ortho-carborane 3 possesses a possible property as a Raman probe, it was difficult to measure Raman imaging in the cell due to its poor water solubility. In fact, ortho-carborane derivative 6, which internally has an alkyne moiety, exhibited very weak Raman signals of the CC stretching and the B-H stretching vibrations were barely detected at a 400 ppm boron concentration in HeLa cells. In contrast, closo-dodecaborate derivatives such as BSH (5) were found to be a potential Raman imaging probe cluster for target molecules in the cell. BSH-conjugated cholesterol 7 (BSH-Chol) was synthesized and used in Raman imaging in cells. Raman imaging and spectral analysis revealed that BSH-based Raman tags provide a versatile platform for quantitative Raman imaging.

Original languageEnglish
Pages (from-to)23973-23978
Number of pages6
JournalRSC Advances
Volume9
Issue number41
DOIs
Publication statusPublished - 2019
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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