Radioimmunoassay for Estradiol 3-Sulfate 17-Glucuronide in Serum Using Specific Antiserum without Deconjugation 1)

Toshio Nambara, Toshifumi Niwa, Kazutake Shimada

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Direct radioimmunoassay for the determination of estradiol 3-sulfate 17-glucuronide in serum using highly specific antiserum without prior deconjugation has been developed. Antiserum was raised in the rabbit by immunization with antigen in which the steroid hapten is coupled to a carrier protein through the C-6 position. The proposed method employing this specific antiserum only [needs a pretreatment on a Sep-pak C 18 cartridge. A simple and reliable assay procedure for the [determination of estradiol 3-sulfate 17-glu-curonide was established and applied to peripheral blood during pregnancy.

Original languageEnglish
Pages (from-to)370-374
Number of pages5
JournalJapanese Journal of Clinical Chemistry
Volume14
Issue number6
DOIs
Publication statusPublished - 1986 Jan 1

ASJC Scopus subject areas

  • Clinical Biochemistry

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