Radioimmunoassay for detecting pro-Leu-enkephalins in tissue extracts: Purification and identification of [Arg6]-Leu-enkephalin in porcine pituitary

Kenji Kangawa, Kensaku Mizuno, Naoto Minamino, Hisayuki Matsuo

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

As a tool for detecting pro-Leu-enkephalins related to α-neo-endorphin and dynorphin in their purification steps from tissue extracts, we have developed a highly sensitive radioimmunoassay for [Arg6]-Leu-enkephalin, which is involved as a common N-terminal hexapeptide both in α-neo-endorphin and dynorphin. By utilizing this radioimmunoassay, we have purified from acid extracts of porcine pituitaries an immunoreactive peptide to the homogeneity, which has been identified as Tyr-Gly-Gly-Phe-Leu-Arg ([Arg6]-Leu-enkephalin).

Original languageEnglish
Pages (from-to)1467-1474
Number of pages8
JournalTopics in Catalysis
Volume95
Issue number4
DOIs
Publication statusPublished - 1980 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Radioimmunoassay for detecting pro-Leu-enkephalins in tissue extracts: Purification and identification of [Arg<sup>6</sup>]-Leu-enkephalin in porcine pituitary'. Together they form a unique fingerprint.

Cite this