Radioimmunoassay for detecting pro-Leu-enkephalins in tissue extracts: Purification and identification of [Arg6]-Leu-enkephalin in porcine pituitary

Kenji Kangawa; Kensaku Mizuno; Naoto Minamino; Hisayuki Matsuo

doi:10.1016/S0006-291X(80)80062-3

Radioimmunoassay for detecting pro-Leu-enkephalins in tissue extracts: Purification and identification of [Arg⁶]-Leu-enkephalin in porcine pituitary

Kenji Kangawa, Kensaku Mizuno, Naoto Minamino, Hisayuki Matsuo

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

As a tool for detecting pro-Leu-enkephalins related to α-neo-endorphin and dynorphin in their purification steps from tissue extracts, we have developed a highly sensitive radioimmunoassay for [Arg⁶]-Leu-enkephalin, which is involved as a common N-terminal hexapeptide both in α-neo-endorphin and dynorphin. By utilizing this radioimmunoassay, we have purified from acid extracts of porcine pituitaries an immunoreactive peptide to the homogeneity, which has been identified as Tyr-Gly-Gly-Phe-Leu-Arg ([Arg⁶]-Leu-enkephalin).

Original language	English
Pages (from-to)	1467-1474
Number of pages	8
Journal	Topics in Catalysis
Volume	95
Issue number	4
DOIs	https://doi.org/10.1016/S0006-291X(80)80062-3
Publication status	Published - 1980 Jan 1
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Biophysics
Molecular Biology

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10.1016/S0006-291X(80)80062-3

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title = "Radioimmunoassay for detecting pro-Leu-enkephalins in tissue extracts: Purification and identification of [Arg6]-Leu-enkephalin in porcine pituitary",

abstract = "As a tool for detecting pro-Leu-enkephalins related to α-neo-endorphin and dynorphin in their purification steps from tissue extracts, we have developed a highly sensitive radioimmunoassay for [Arg6]-Leu-enkephalin, which is involved as a common N-terminal hexapeptide both in α-neo-endorphin and dynorphin. By utilizing this radioimmunoassay, we have purified from acid extracts of porcine pituitaries an immunoreactive peptide to the homogeneity, which has been identified as Tyr-Gly-Gly-Phe-Leu-Arg ([Arg6]-Leu-enkephalin).",

author = "Kenji Kangawa and Kensaku Mizuno and Naoto Minamino and Hisayuki Matsuo",

year = "1980",

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doi = "10.1016/S0006-291X(80)80062-3",

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T1 - Radioimmunoassay for detecting pro-Leu-enkephalins in tissue extracts

T2 - Purification and identification of [Arg6]-Leu-enkephalin in porcine pituitary

AU - Kangawa, Kenji

AU - Mizuno, Kensaku

AU - Minamino, Naoto

AU - Matsuo, Hisayuki

PY - 1980/1/1

Y1 - 1980/1/1

N2 - As a tool for detecting pro-Leu-enkephalins related to α-neo-endorphin and dynorphin in their purification steps from tissue extracts, we have developed a highly sensitive radioimmunoassay for [Arg6]-Leu-enkephalin, which is involved as a common N-terminal hexapeptide both in α-neo-endorphin and dynorphin. By utilizing this radioimmunoassay, we have purified from acid extracts of porcine pituitaries an immunoreactive peptide to the homogeneity, which has been identified as Tyr-Gly-Gly-Phe-Leu-Arg ([Arg6]-Leu-enkephalin).

AB - As a tool for detecting pro-Leu-enkephalins related to α-neo-endorphin and dynorphin in their purification steps from tissue extracts, we have developed a highly sensitive radioimmunoassay for [Arg6]-Leu-enkephalin, which is involved as a common N-terminal hexapeptide both in α-neo-endorphin and dynorphin. By utilizing this radioimmunoassay, we have purified from acid extracts of porcine pituitaries an immunoreactive peptide to the homogeneity, which has been identified as Tyr-Gly-Gly-Phe-Leu-Arg ([Arg6]-Leu-enkephalin).

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Radioimmunoassay for detecting pro-Leu-enkephalins in tissue extracts: Purification and identification of [Arg⁶]-Leu-enkephalin in porcine pituitary

Abstract

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