Radiation dose-rate effect on mutation induction in spleen and liver of gpt delta mice

Naohito Okudaira, Yoshihiko Uehara, Kazuo Fujikawa, Nao Kagawa, Akira Ootsuyama, Toshiyuki Norimura, Ken Ichi Saeki, Takehiko Nohmi, Ken Ichi Masumura, Tsuneya Matsumoto, Yoichi Oghiso, Kimio Tanaka, Kazuaki Ichinohe, Shingo Nakamura, Satoshi Tanaka, Tetsuya Ono

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The effect of dose rate on radiation-induced mutations in two somatic tissues, the spleen and liver, was examined in transgenic gpt delta mice. These mice can be used for the detection of deletion-type mutations, and these are the major type of mutation induced by radiation. The dose rates examined were 920 mGy/min, 1 mGy/min and 12.5 μGy/min. In both tissues, the number of mutations increased with increasing dose at each of the three dose rates examined. The mutation induction rate was dependent on the dose rate. The mutation induction rate was higher in the spleen than in the liver at the medium dose rate but was similar in the two tissues at the high and low dose rates. The mutation induction rate in the liver did not show much change between the medium and low dose rates. Analysis of the molecular nature of the mutations indicated that 2-to 1,000-bp deletion mutations were specifically induced by radiation in both tissues after high-and low-dose-rate irradiation. The occurrence of deletion mutation without any sequence homology at the break point was elevated in spleen after high-dose-rate irradiation. The results indicate that the mutagenic effects of radiation in somatic tissues are dependent on dose rate and that there is some variability between tissues.

Original languageEnglish
Pages (from-to)138-147
Number of pages10
JournalRadiation Research
Volume173
Issue number2
DOIs
Publication statusPublished - 2010 Feb

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

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