Rad50 is involved in MMS-induced recombination between homologous chromosomes in mitotic cells

Yuji Tomizawa, Ayako Ui, Fumitoshi Onoda, Hideaki Ogiwara, Shusuke Tada, Takemi Enomoto, Masayuki Seki

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The structural maintenance of chromosomes (SMC) family proteins (Smc1-Smc6) typically consist of two coiled-coil domains, an amino-terminal head domain, and a carboxyl-terminal tail domain. Rad50, a component of the Mre11/Rad50/Xrs2 (MRX) complex, has a similar domain structure to the SMC proteins. In Saccharomyces cerevisiae, the MRX complex appears to be essential for recombination between homologous chromosomes in meiotic cells, but not in cells undergoing vegetative growth. Here we provide for the first time evidence that Rad50, like Smc6, is required for the induction of recombination between homologous chromosomes in cells in the vegetative growth state upon exposure to methyl methanesulfonate. However, UV-induced recombination between homologous chromosomes is intact in both rad50 and smc6-56 mutant cells.

Original languageEnglish
Pages (from-to)157-160
Number of pages4
JournalGenes and Genetic Systems
Volume82
Issue number2
DOIs
Publication statusPublished - 2007

Keywords

  • Homologous recombination
  • Mre11; Rad50; Xrs2; MRX
  • Smc5
  • Smc6

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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