RAB5 activation is required for multiple steps in arabidopsis thaliana root development

Takeshi Inoue, Yuki Kondo, Satoshi Naramoto, Akihiko Nakano, Takashi Ueda

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Rab GTPases regulate the tethering and fusion of transport vesicles to target membranes in membrane trafficking by acting as a molecular switch, cycling between GDP-and GTP-bound states. RAB5 is a member of the Rab GTPase family, the members of which have been shown to perform various functions in the endocytic pathway, including the regulation of endosomal fusion and motility in animal cells. RAB5-mediated endosomal trafficking has also been found to play important roles in various higher order plant functions, which include the regulation of the polar transport of auxin and responses to environmental conditions. The regulatory mechanisms and functions of plant RAB5 have also been investigated at the molecular and cellular levels. However, the significance of RAB5 activity at the tissue and organ levels has hardly been investigated thus far. In the present study, we examined the effect of a mutation in VPS9a, which encodes the sole guanine nucleotide exchange factor for all RAB5s in the vegetative stages of Arabidopsis thaliana. We found that multiple developmental processes were impaired in the mutant plants, including the growth and pattern formation of the roots and establishment of auxin maxima. Our results indicate that RAB5 plays distinctive pivotal roles in the development of plants.

Original languageEnglish
Pages (from-to)1648-1659
Number of pages12
JournalPlant and Cell Physiology
Volume54
Issue number10
DOIs
Publication statusPublished - 2013 Oct
Externally publishedYes

Keywords

  • Arabidosis thaliana
  • Development
  • Endosomal traffic
  • Guanine nucleotide exchange factor
  • RAB5

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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