R76 in transmembrane domain 3 of the aspartate:alanine transporter AspT is involved in substrate transport

Satomi Suzuki, Kei Nanatani, Keietsu Abe

Research output: Contribution to journalArticlepeer-review

Abstract

The L-aspartate:L-alanine antiporter of Tetragenococcus halophilus (AspT) possesses an arginine residue (R76) within the GxxxG motif in the central part of transmembrane domain 3 (TM3) - a residue that has been estimated to transport function. In this study, we carried out amino acid substitutions of R76 and used proteoliposome reconstitution for analyzing the transport function of each substitution. Both L-aspartate and L-alanine transport assays showed that R76K has higher activity than the AspT-WT (R76), whereas R76D and R76E have lower activity than the AspT-WT. These results suggest that R76 is involved in AspT substrate transport.

Original languageEnglish
Pages (from-to)744-747
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume80
Issue number4
DOIs
Publication statusPublished - 2016 Jan 1

Keywords

  • Amino acid transporter
  • GxxxG
  • Liposome
  • Substrate transport
  • Transport activity

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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